Flavor notes derived from Maillard reaction of glucose with individual amino acids

Kam Huey Wong , Suraini Abdul Aziz , and Suhaila Mohamed at Universiti Putra, Malaysia reported in International Journal of Food Science and Technology Vol 43-Issue 9, Sept 2008 that individual amino acids can be used to generate specific flavor note through Maillard reaction with glucose.

The reaction condition used for all the reaction experiments:

Glucose monohydrate 10 mmol
Individual amino acid: 3.3 mmol
in 20 ml water.
PH for each reaction varied depending upon the amino acid and experimental design.
The reaction solution was heated at various temperatures for a various period of time.

Reacted solutions were evaluated against standard flavorings (bouillon, meaty, soy sauce, smoky, malty, caramel, beany, chicken, fruity, pandan, vanilla, seafood, chocolate, coffee, buttery to characterize flavor note in each reaction solution.

Taste of a reaction solution was evaluated against solutions of pure chemicals: Sweet (sucrose), sour (citric acid), salty(sodium chloride), bitter (caffeine), and umami (monosodium glutamate or MSG. (Note flavorists may use different chemicals to characterize the taste.

Study findings

Color and odor of Maillard products of amino acids and glucose formed after heating for 14 and/or 24 h at original pH

Amino acidOriginal measured pH14 and/or 24 hours
ColourOdour
Alanine5.6BrownFruity** (Persimmon), pleasant/sweet**, flowery**,
Arginine5.3Light yellowNone, bitter taste*
Aspartic acid3.0Very light brownFruity** (ciku, fresh dates), pleasant/sweet**
Cysteine4.5Light yellowSulphury**
Glutamic acid3.2No changeNone, sour taste**
Glycine5.7BrownCaramel-like**, pleasant/sweet*
Histidine4.0Light yellowNone, slight sweet-sour taste
Isoleucine5.8BrownBurnt*, caramel-like*
Leucine5.7BrownBurnt**, caramel-like*
Lysine5.4BrownPleasant/sweet*, caramel-like**, bitter taste
Methionine5.6BrownFried potatoes, prawn cracker*
Threonine5.6Light brownPleasant/sweet**, astringent sweet taste
Serine5.5BrownFruity* (slightly ciku, fresh dates), pleasant/sweet*
Proline5.7Light brownFlowery**, pleasant/sweet**, pandan**, slightly persimmon, bitter taste
Phenylalanine5.4BrownFlowery** (dried roses), Almond, bitter taste
Tyrosine5.6BrownFlowery** (slightly dried roses), sweet taste
Valine5.6BrownCaramel-like**, bitter taste
None (glucose), control5.4No changeNone

Color and odor of Maillard products of amino acids and glucose formed after heating for 14 and/or 24 h at original pH

Amino acidOriginal measured pH14 and/or 24 hours
ColourOdour
Alanine5.6BrownFruity** (Persimmon), pleasant/sweet**, flowery**,
Arginine5.3Light yellowNone, bitter taste*
Aspartic acid3.0Very light brownFruity** (ciku, fresh dates), pleasant/sweet**
Cysteine4.5Light yellowSulphury**
Glutamic acid3.2No changeNone, sour taste**
Glycine5.7BrownCaramel-like**, pleasant/sweet*
Histidine4.0Light yellowNone, slight sweet-sour taste
Isoleucine5.8BrownBurnt*, caramel-like*
Leucine5.7BrownBurnt**, caramel-like*
Lysine5.4BrownPleasant/sweet*, caramel-like**, bitter taste
Methionine5.6BrownFried potatoes, prawn cracker*
Threonine5.6Light brownPleasant/sweet**, astringent sweet taste
Serine5.5BrownFruity* (slightly ciku, fresh dates), pleasant/sweet*
Proline5.7Light brownFlowery**, pleasant/sweet**, pandan**, slightly persimmon, bitter taste
Phenylalanine5.4BrownFlowery** (dried roses), Almond, bitter taste
Tyrosine5.6BrownFlowery** (slightly dried roses), sweet taste
Valine5.6BrownCaramel-like**, bitter taste
None (glucose), control5.4No changeNone

Color and odor of Maillard products of amino acids and glucose formed after heating for 24 h at starting pH 5.2 and controlled at pH 5.2 ± 0.1 conditions

Amino acidColourOdour
AlanineBrownFruity** (ciku, fresh dates), pleasant/sweet**, flowery**
ArginineBrownPleasant/sweet**, fruity*, sour*
Aspartic acidBrownFruity (ciku, fresh dates), pleasant/sweet**, caramel-like**
CysteineVery light brownSulphury**, slightly meaty, boiled chickpeas
Glutamic acidBrownNone, sour umami taste,
GlycineBrownPleasant/sweet**, flowery*
HistidineLight yellowNone, sour taste
IsoleucineBrownBurnt**, caramel-like**
LeucineBrownBurnt**, caramel-like*, biscuit-like
LysineBrownPleasant/sweet**, pandan*,bitter taste
MethionineBrownFried potatoes, prawn crackers*
ThreonineLight brownPleasant/sweet**, fruity**
SerineBrownPleasant/sweet**
ProlineBrownFlowery*, pleasant/sweet*, pandan**
PhenylalanineBrown (cloudy)Flowery** (roses), almond, Mimusops elengi flower**
TyrosineBrownFlowery*, fruity*, pleasant/sweet, tea-like
ValineBrownCaramel-like**, biscuit-like, malty, chocolate, bitter taste
None (glucose)No changeNone

Color and odor of Maillard products of amino acids and glucose formed after heating for 1 h in the presence of 6 m HCl (pH < 0.1)

Amino acidColourOdour
AlanineBrownPleasant/sweet**, fruity* (ciku, fresh dates), caramel-like**, slightly burnt**
ArginineBrownCaramel-like*, burnt**, slight sweet/pleasant*
Aspartic AcidBrownPleasant/sweet**, fruity (ciku, fresh dates), caramel-like**
CysteineDark brownSulphury**, meaty*
Glutamic AcidBrownPleasant/sweet**, caramel-like**, burnt**
GlycineBrownPleasant/sweet**, caramel-like**, burnt**
HistidineBrownCaramel-like**, burnt*
IsoleucineBrownBurnt**, coffee-like*, prune
LeucineBrownBurnt*, coffee-like*, caramel-like*, malty*
LysineBrownPleasant/sweet**, cardboard, Herbal tea*
MethionineBrownMeaty**, sulphury**, fried potatoes*
ThreonineBrownPleasant/sweet**, fruity** (ciku, fresh dates), flowery*, caramel-like**
SerineBrownSlightly burnt**, pleasant/sweet*
ProlineBrownFlowery**, pleasant/sweet**, pandan*, slightly alkaline
PhenylalanineBrownFlowery** (roses)
TyrosineBrownPleasant/sweet*, caramel-like*, burnt**
ValineBrownPleasant/sweet**, caramel-like**, burnt*, malty

Comparison of color and odor of Maillard products of amino acids and glucose heated and maintained at pH 5.2 ± 0.1 served at room temperature and 60 °C

Served at room temperatureServed at 60 °C
Amino acidColourOdourColourOdour
ArginineBrownPleasant/sweet**, fruity*, sour*BrownPleasant/sweet**, caramel-like**, fruity, sour
Glutamic acidBrownNone**, fruity sour umami tasteBrownPleasant/sweet**, caramel-like*, biscuit-like*
GlycineBrownPleasant/sweet**, flowery*BrownPleasant/sweet**, flowery*, caramel-like**
HistidineLight yellowNone**, sour tasteLight yellowPleasant/sweet**, caramel-like*
LysineBrownPleasant/sweet**, pandan*BrownPleasant/sweet**, pandan*, flowery*
ThreonineVery light brownPleasant/sweet**, fruity**Very light brownPleasant/sweet**, fruity*
SerineBrownPleasant/sweet**BrownPleasant/sweet**, caramel-like**
TyrosineBrownFlowery*, fruity*, Pleasant/sweet*, tea-likeBrownFlowery** (roses), fruity, Pleasant/sweet, tea-like
ValineBrownCaramel-like**, biscuit-like, malty, chocolateBrownCaramel-like**, biscuit-like, malty, chocolate, burnt**

Those who are interested in details may download a FTP file of the report.